P57 (acLys278) antibody

Catalog No. ABIN7212628

The Rabbit Polyclonal anti-P57 antibody is suitable to detect P57 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for P57 (acLys278) antibody (ABIN7212628)

Target: P57

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Product Details

Binding Specificity: acLys278

Purpose: Rabbit Anti-p57 (Acetyl Lys278) Polyclonal Antibody

Specificity: Acetyl-p57 (K278) Polyclonal Antibody detects endogenous levels of p57 protein only when acetylated at K278.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human p57 around the acetylation site of K278

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: P57

Background: CDKN1C, KIP2, Cyclin-dependent kinase inhibitor 1C, Cyclin-dependent kinase inhibitor p57, p57Kip2CDKN1C is imprinted, with preferential expression of the maternal allele. Cyclin dependent kinase inhibitor 1C is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in CDKN1C are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that CDKN1C is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for CDKN1C.

Molecular Weight: 33kD

Gene ID: 1028

UniProt: P49918