P57 (AA 1-200) antibody

Catalog No. ABIN7868692

The Mouse Monoclonal anti-P57 antibody (Clone KIP2-7639) (ABIN7868692) specifically detects P57 in IHC (p). The antibody is reactive with Human samples.

Quick Overview for P57 (AA 1-200) antibody (ABIN7868692)

Target: P57

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: KIP2-7639

Product Details

Binding Specificity: AA 1-200

Purpose: p57 Antibody / p57Kip2 (azide and preservative free)

Purification: Protein A/G affinity

Immunogen: A recombinant fragment (within amino acids 1-200) of human p57Kip2 protein was used as the immunogen for the p57 antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal dilution of the p57 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C

Storage Comment: Aliquot the p57 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details

Target: P57

Background: Recognizes a protein of 57 kDa, identified as p57Kip2. It shows no cross-reaction with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. Anti-p57 has been used as an aide in identification of complete hydatidiform mole (CHM) (no nuclear labeling of cytotrophoblasts and stromal cells) from partial hydatidiform mole (PHM) in which both cytotrophoblasts and stromal cells stain. The histological differentiation of complete mole, partial mole, and hydropic spontaneous abortion is problematic. Most complete hydatidiform moles are diploid, whereas most partial moles are triploid. Ploidy studies will identify partial moles, but will not differentiate complete moles from non-molar gestations. Complete moles carry a high risk of persistent disease and choriocarcinoma, while partial moles have a very low risk. In normal placenta, many cytotrophoblast nuclei and stromal cells are labeled with this antibody. Similar findings apply to PHM and hydropic abortus tissues. Intervillous trophoblastic islands (IVTIs) demonstrate nuclear labeling in all three entities and serve as an internal control.

UniProt: P49918