PAX5 antibody (AA 1-80)

Catalog No. ABIN7216458

The Rabbit Polyclonal anti-PAX5 antibody (ABIN7216458) specifically detects PAX5 in WB and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for PAX5 antibody (AA 1-80) (ABIN7216458)

Target: PAX5 (Paired Box 5 (PAX5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAX5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PAX5 Antibody

Binding Specificity: AA 1-80

Purpose: Rabbit Anti-Pax-5 Polyclonal Antibody

Specificity: Pax-5 Polyclonal Antibody detects endogenous levels of Pax-5 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human Pax-5 at AA range: 1-80

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PAX5

Target: PAX5 (Paired Box 5 (PAX5))

Alternative Name: Pax-5

Background: PAX5, Paired box protein Pax-5, B-cell-specific transcription factor, BSAPPAX5 encodes a member of the paired box (PAX) family of transcription factors. The central feature of PAX5 family is a novel, highly conserved DNA-binding motif, known as the paired box. Paired box transcription factors are important regulators in early development, and alterations in the expression of PAX5 are thought to contribute to neoplastic transformation. PAX5 encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the paired box 5 may also play a role in neural development and spermatogenesis. PAX5 is located at 9p13, which is involved in t(9,14)(p13,q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of PAX5 contributes to the pathogenesis of these lymphomas. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 40kD

Gene ID: 5079

UniProt: Q02548