PAK3 antibody (pSer154)

Catalog No. ABIN7222928

The Rabbit Polyclonal anti-PAK3 antibody (ABIN7222928) specifically detects PAK3 in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PAK3 antibody (pSer154) (ABIN7222928)

Target: PAK3

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-PAK3 Antibody

Binding Specificity: pSer154

Purpose: Rabbit Anti-PAKβ (phospho Ser154) Polyclonal Antibody

Specificity: Phospho-PAKβ (S154) Polyclonal Antibody detects endogenous levels of PAKβ protein only when phosphorylated at S154.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PAKbeta Phospho-Ser154

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PAK3

Target: PAK3

Alternative Name: PAKbeta

Background: PAK3, OPHN3, Serine/threonine-protein kinase PAK 3, Beta-PAK, Oligophrenin-3, p21-activated kinase 3, PAK-3The protein encoded by PAK3 is a serine-threonine kinase and forms an activated complex with GTP-bound RAS-like P21, CDC2 and RAC1. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in PAK3 are the cause of non-syndromic mental retardation X-linked type 30 (MRX30), also called X-linked mental retardation type 47 (MRX47). Alternatively spliced transcript variants encoding different isoforms have been identified.

Molecular Weight: 72kD

Gene ID: 5063

UniProt: O75914