Paxillin antibody (pTyr31)

Catalog No. ABIN7213106

The Rabbit Polyclonal anti-Paxillin antibody (ABIN7213106) specifically detects Paxillin in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Paxillin antibody (pTyr31) (ABIN7213106)

Target: Paxillin (PXN)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Paxillin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Paxillin Antibody

Binding Specificity: pTyr31

Purpose: Rabbit Anti-Paxillin (phospho Tyr31) Polyclonal Antibody

Specificity: Phospho-Paxillin (Y31) Polyclonal Antibody detects endogenous levels of Paxillin protein only when phosphorylated at Y31.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Paxillin Phospho-Tyr31

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Paxillin

Target: Paxillin (PXN)

Alternative Name: Paxillin

Background: PXN, PaxillinPXN encodes a cytoskeletal protein (paxillin) involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for PXN.These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445).

Molecular Weight: 68kD

Gene ID: 5829

UniProt: P49023

Pathways: MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling