Phospholipase D2 antibody (pTyr169)

Catalog No. ABIN7223198

The Rabbit Polyclonal anti-Phospholipase D2 antibody is suitable to detect Phospholipase D2 in samples from Human, Rat and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for Phospholipase D2 antibody (pTyr169) (ABIN7223198)

Target: Phospholipase D2 (PLD2)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase D2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Phospholipase D2 Antibody

Binding Specificity: pTyr169

Purpose: Rabbit Anti-PC-PLD2 (phospho Tyr169) Polyclonal Antibody

Specificity: Phospho-PC-PLD2 (Y169) Polyclonal Antibody detects endogenous levels of PC-PLD2 protein only when phosphorylated at Y169.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PC-PLD2 Phospho-Tyr169

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Phospholipase D2

Target: Phospholipase D2 (PLD2)

Alternative Name: PC-PLD2

Background: PLD2, Phospholipase D2, PLD 2, hPLD2, Choline phosphatase 2, PLD1C, Phosphatidylcholine-hydrolyzing phospholipase D2The protein encoded by PLD2 (phospholipase D2) catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for PLD2.

Molecular Weight: 95kD

Gene ID: 5338

UniProt: O14939

Pathways: RTK Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling