PBK antibody

Catalog No. ABIN7223548

The Rabbit Polyclonal anti-PBK antibody is suitable to detect PBK in samples from Human and Monkey. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for PBK antibody (ABIN7223548)

Target: PBK (PDZ Binding Kinase (PBK))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PBK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PBK Antibody

Purpose: Rabbit Anti-PBK Polyclonal Antibody

Specificity: PBK Polyclonal Antibody detects endogenous levels of PBK protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PBK around the non-phosphorylation site of T9

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PBK

Target: PBK (PDZ Binding Kinase (PBK))

Alternative Name: PBK

Background: PBK, TOPK, Lymphokine-activated killer T-cell-originated protein kinase, Cancer/testis antigen 84, CT84, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, Spermatogenesis-related protein kinase, SPK, T-LAK cell-originated protein kinasPBK (PDZ binding kinase) encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of PBK has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants.

Molecular Weight: 36kD

Gene ID: 55872

UniProt: Q96KB5