PPHLN1 antibody (AA 40-120)

Catalog No. ABIN7216496

The Rabbit Polyclonal anti-PPHLN1 antibody is suitable to detect PPHLN1 in samples from Human and Mouse. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for PPHLN1 antibody (AA 40-120) (ABIN7216496)

Target: PPHLN1 (Periphilin 1 (PPHLN1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPHLN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-PPHLN1 Antibody

Binding Specificity: AA 40-120

Purpose: Rabbit Anti-Periphilin 1 Polyclonal Antibody

Specificity: Periphilin 1 Polyclonal Antibody detects endogenous levels of Periphilin 1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Periphilin 1 at AA range: 40-120

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PPHLN1

Target: PPHLN1 (Periphilin 1 (PPHLN1))

Alternative Name: Periphilin 1

Background: PPHLN1, HSPC206, HSPC232, Periphilin-1, Gastric cancer antigen Ga50Periphilin 1 encoded by PPHLN1 is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. Periphilin 1 interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of periphilin 1 suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.

Molecular Weight: 53kD

Gene ID: 51535

UniProt: Q8NEY8