PFKL antibody (AA 300-380)

Catalog No. ABIN7216514

The Rabbit Polyclonal anti-PFKL antibody is suitable to detect PFKL in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IHC.

Quick Overview for PFKL antibody (AA 300-380) (ABIN7216514)

Target: PFKL (Phosphofructokinase, Liver (PFKL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-PFKL Antibody

Binding Specificity: AA 300-380

Purpose: Rabbit Anti-PFK-2 liv/tes Polyclonal Antibody

Specificity: PFK-2 liv/tes Polyclonal Antibody detects endogenous levels of PFK-2 liv/tes protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human PFK-2 liv/tes at AA range: 300-380

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300,ELISA 1:2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PFKL

Target: PFKL (Phosphofructokinase, Liver (PFKL))

Alternative Name: PFK-2 liv/tes

Background: PFKFB1, F6PK, PFRX, 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1, 6PF-2-K/Fru-2,6-P2ase 1, PFK/FBPase 1, 6PF-2-K/Fru-2,6-P2ase liver isozyme, PFKFB4, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4, 6PF-2-K/Fru-2,6-P2ase 4,PFKFB1 encodes a member of the family of bifunctional 6-phosphofructo-2-kinase: fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 is thought to regulate glucose homeostasis. Multiple alternatively spliced transcript variants have been found for PFKFB1.

Molecular Weight: 54kD

Gene ID: 5207, 5210

UniProt: P16118, Q16877

Pathways: Negative Regulation of Hormone Secretion, Warburg Effect