PHF1 antibody (AA 320-400)

Catalog No. ABIN7216528

This Rabbit Polyclonal antibody specifically detects PHF1 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PHF1 antibody (AA 320-400) (ABIN7216528)

Target: PHF1 (PHD Finger Protein 1 (PHF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PHF1 Antibody

Binding Specificity: AA 320-400

Purpose: Rabbit Anti-PHF1 Polyclonal Antibody

Specificity: PHF1 Polyclonal Antibody detects endogenous levels of PHF1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human PHF1 at AA range: 320-400

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PHF1

Target: PHF1 (PHD Finger Protein 1 (PHF1))

Alternative Name: PHF1

Background: PHF1, PCL1, PHD finger protein 1, Protein PHF1, Polycomb-like protein 1, hPCl1PHF1 encodes a Polycomb group protein. PHD finger protein 1 is a component of a histone H3 lysine-27 (H3K27)-specific methyltransferase complex, and functions in transcriptional repression of homeotic genes. PHD finger protein 1 is also recruited to double-strand breaks, and reduced protein levels results in X-ray sensitivity and increased homologous recombination. Multiple transcript variants encoding different isoforms have been found for PHF1.

Molecular Weight: 62kD

Gene ID: 5252

UniProt: O43189