PIK3R2 antibody (pTyr464)

Catalog No. ABIN7223152

The Rabbit Polyclonal anti-PIK3R2 antibody is suitable to detect PIK3R2 in samples from Human, Rat and Mouse. It has been validated for WB and ELISA.

Quick Overview for PIK3R2 antibody (pTyr464) (ABIN7223152)

Target: PIK3R2 (PI3K p85b) (Phosphoinositide 3 Kinase, p85 beta (PI3K p85b))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3R2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PIK3R2 Antibody

Binding Specificity: pTyr464

Purpose: Rabbit Anti-PI 3-kinase p85β (phospho Tyr464) Polyclonal Antibody

Specificity: Phospho-PI 3-kinase p85β (Y464) Polyclonal Antibody detects endogenous levels of PI 3-kinase p85β protein only when phosphorylated at Y464.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PI 3-kinase p85beta Phospho-Tyr464

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PIK3R2

Target: PIK3R2 (PI3K p85b) (Phosphoinositide 3 Kinase, p85 beta (PI3K p85b))

Alternative Name: PI 3-kinase p85beta

Background: PIK3R2, Phosphatidylinositol 3-kinase regulatory subunit beta, PI3-kinase regulatory subunit beta, PI3K regulatory subunit beta, PtdIns-3-kinase regulatory subunit beta, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta, PI3-kinaPhosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by PIK3CG (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma) is a class I catalytic subunit of PI3K. Like other class I catalytic subunits (p110-alpha p110-beta, and p110-delta), the encoded protein binds a p85 regulatory subunit to form PI3K. This gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for PIK3CG.

Molecular Weight: 85kD

Gene ID: 5296

UniProt: O00459

Pathways: VEGF Signaling, BCR Signaling