PIM1 antibody (pTyr309)

Catalog No. ABIN7223146

The Rabbit Polyclonal anti-PIM1 antibody is suitable to detect PIM1 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for PIM1 antibody (pTyr309) (ABIN7223146)

Target: PIM1 (Pim-1 Oncogene (PIM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PIM1 Antibody

Binding Specificity: pTyr309

Purpose: Pim-1 (phospho Tyr309) Polyclonal Antibody

Specificity: Phospho-Pim-1 (Y309) Polyclonal Antibody detects endogenous levels of Pim-1 protein only when phosphorylated at Y309.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Pim-1 Phospho-Tyr309

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PIM1

Target: PIM1 (Pim-1 Oncogene (PIM1))

Alternative Name: Pim-1

Background: Rabbit Anti-Pim-1 (phospho Tyr309) Polyclonal Antibody,PIM1, Serine/threonine-protein kinase pim-1,The protein encoded by PIM1 (Pim-1 proto-oncogene, serine/threonine kinase) belongs to the Ser/Thr protein kinase family, and PIM subfamily. PIM1 is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons.,serine threonine-protein kinase pim-1

Molecular Weight: observerd band 45kDa

Gene ID: 5292

UniProt: P11309

Pathways: Glycosaminoglycan Metabolic Process