POLE2 antibody (Internal Region)

Catalog No. ABIN7217682

The Rabbit Polyclonal anti-POLE2 antibody has been validated for WB and ELISA. It is suitable to detect POLE2 in samples from Human.

Quick Overview for POLE2 antibody (Internal Region) (ABIN7217682)

Target: POLE2 (Polymerase (DNA Directed), epsilon 2 (p59 Subunit) (POLE2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLE2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-POLE2 Antibody

Binding Specificity: Internal Region

Purpose: DNA pol ε B Polyclonal Antibody

Specificity: DNA pol ε B Polyclonal Antibody detects endogenous levels of DNA pol ε B protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human DNA pol epsilon B

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for POLE2

Target: POLE2 (Polymerase (DNA Directed), epsilon 2 (p59 Subunit) (POLE2))

Alternative Name: DNA pol epsilon B

Background: Rabbit Anti-DNA pol ε B Polyclonal Antibody,POLE2, DPE2, DNA polymerase epsilon subunit 2, DNA polymerase II subunit 2, DNA polymerase epsilon subunit B,POLE2 (Polymerase (DNA) Epsilon 2, Accessory Subunit) is a Protein Coding gene. Among its related pathways are Metabolism and Cell Cycle, Mitotic. Gene Ontology (GO) annotations related to this gene include DNA-directed DNA polymerase activity. Participates in DNA repair and in chromosomal DNA replication.,DNA polymerase epsilon subunit 2

Molecular Weight: observerd band 59kDa

Gene ID: 5427

UniProt: P56282

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA