POLH antibody

Catalog No. ABIN7230724

This Rabbit Polyclonal antibody specifically detects POLH in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for POLH antibody (ABIN7230724)

Target: POLH (Polymerase (DNA Directed), eta (POLH))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLH antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-POLH Antibody

Purpose: POLH Polyclonal Antibody

Specificity: The antibody detects endogenous levels of POLH protein

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

Immunogen: Synthesized peptide derived from part region of human POLH protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000-1:20000).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for POLH

Target: POLH (Polymerase (DNA Directed), eta (POLH))

Alternative Name: POLH

Background: Rabbit Anti-POLH Polyclonal Antibody,DNA polymerase eta, RAD30 homolog A, Xeroderma pigmentosum variant type protein,POLH encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA, when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene.,POLH

Molecular Weight: observerd band 78kDa

Gene ID: 5429

UniProt: Q9Y253

Pathways: DNA Damage Repair