POLE4 antibody (AA 30-110)

Catalog No. ABIN7223666

The Rabbit Polyclonal anti-POLE4 antibody is suitable to detect POLE4 in samples from Human and Mouse. It has been validated for ELISA, IHC and IF.

Quick Overview for POLE4 antibody (AA 30-110) (ABIN7223666)

Target: POLE4 (Polymerase (DNA-Directed), epsilon 4 (p12 Subunit) (POLE4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLE4 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-POLE4 Antibody

Binding Specificity: AA 30-110

Purpose: Rabbit Anti-DNA pol ε 4 Polyclonal Antibody

Specificity: DNA pol ε 4 Polyclonal Antibody detects endogenous levels of DNA pol ε 4 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human DNA pol epsilon 4 at AA range: 30-110

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for POLE4

Target: POLE4 (Polymerase (DNA-Directed), epsilon 4 (p12 Subunit) (POLE4))

Alternative Name: DNA pol epsilon 4

Background: POLE4, DNA polymerase epsilon subunit 4, DNA polymerase II subunit 4, DNA polymerase epsilon subunit p12POLE4 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.

Molecular Weight: 12kD

Gene ID: 56655

UniProt: Q9NR33

Pathways: DNA Damage Repair