PRCP antibody

Catalog No. ABIN7230534

The Rabbit Polyclonal anti-PRCP antibody (ABIN7230534) specifically detects PRCP in WB and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for PRCP antibody (ABIN7230534)

Target: PRCP (Prolylcarboxypeptidase (PRCP))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRCP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PRCP Antibody

Purpose: Rabbit Anti-PCP Polyclonal Antibody

Specificity: The antibody detects endogenous levels of PCP protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human PCP protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PRCP

Target: PRCP (Prolylcarboxypeptidase (PRCP))

Alternative Name: PCP

Background: Lysosomal Pro-X carboxypeptidase, Angiotensinase C, Lysosomal carboxypeptidase C, Proline carboxypeptidase, Prolylcarboxypeptidase, PRCPPRCP encodes a member of the peptidase S28 family of serine exopeptidases. Prolylcarboxypeptidase is proteolytically processed to generate the mature lysosomal prolylcarboxypeptidase. This enzyme cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH , but the enzyme activity is retained with some substrates at neutral pH . This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. A pseudogene of this gene has been identified on chromosome 2. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

Molecular Weight: 54kD

Gene ID: 5547

UniProt: P42785