UHMK1 antibody (AA 331-419) (Cy3)

Catalog No. ABIN716519

Product Details anti-UHMK1 Antibody (Cy3)

Key Features:

• Rabbit anti-CRBN Antibody reliably detects CRBN in human species.
• Polyclonal, unconjugated antibody for ELISA, WB, IHC.
• Highly specific CRBN detection. See validation images.

Target: UHMK1 (U2AF Homology Motif (UHM) Kinase 1 (UHMK1))

Binding Specificity: AA 331-419

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UHMK1 antibody is conjugated to Cy3

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human KIST/UHMK1

Isotype: IgG

Product Specific Information:

What can the CRBN antibody ABIN7165198 be used for? This polyclonal, unconjugated rabbit anti-CRBN antibody is suitable for the detection of cereblon (CRBN) in ELISA, Western blotting and immunohistochemistry. The CRBN antibody has been validated for the human species. Based on the sequence, cross-reactivity with mouse can be assumed. Recombinant human cereblon protein was used as immunogen for production.

What validation data is available for this CRBN antibody? The CRBN antibody currently has three product images demonstrating its performance in IHC and Western blot. Use our high-quality CRBN Antibody to reliably detect Cereblon.

What is the function of CRBN? CRBN is a substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2 or ILF2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. CRBN maintains presynaptic glutamate release and consequently cognitive functions, such as memory and learning, by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons. (UniProt)

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for UHMK1

Target: UHMK1 (U2AF Homology Motif (UHM) Kinase 1 (UHMK1))

Alternative Name: KIST (UHMK1 Products)

Synonyms: UHMK1 antibody, zgc:153241 antibody, KIS antibody, KIST antibody, P-CIP2 antibody, 4732477C12Rik antibody, 4930500M09Rik antibody, AA673513 antibody, AI449218 antibody, AU021979 antibody, Kist antibody, U2AF homology motif kinase 1 antibody, U2AF homology motif (UHM) kinase 1 antibody, UHMK1 antibody, uhmk1 antibody, Uhmk1 antibody

Background:

Synonyms: KIS, KIST, P-CIP2, Serine/threonine-protein kinase Kist, Kinase interacting with stathmin, PAM COOH-terminal interactor protein 2, U2AF homology motif kinase 1, UHMK1

Background: Upon serum stimulation, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. May be involved in trafficking and/or processing of RNA (By similarity).

Gene ID: 127933

UniProt: Q8TAS1