PPP1R16A antibody (AA 340-420)

Catalog No. ABIN7225488

The Rabbit Polyclonal anti-PPP1R16A antibody is suitable to detect PPP1R16A in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for PPP1R16A antibody (AA 340-420) (ABIN7225488)

Target: PPP1R16A (Protein Phosphatase 1, Regulatory Subunit 16A (PPP1R16A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1R16A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PPP1R16A Antibody

Binding Specificity: AA 340-420

Purpose: Rabbit Anti-MYPT3 Polyclonal Antibody

Specificity: MYPT3 Polyclonal Antibody detects endogenous levels of MYPT3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human MYPT3 at AA range: 340-420

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IF/ICC1:50-200,ELISA 1:2000-20000,IHC 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PPP1R16A

Target: PPP1R16A (Protein Phosphatase 1, Regulatory Subunit 16A (PPP1R16A))

Alternative Name: MYPT3

Background: PPP1R16A, MYPT3, Protein phosphatase 1 regulatory subunit 16A, Myosin phosphatase-targeting subunit 3Myosin light chain kinase and phosphatase (MLCP) complexes control the phosphorylation states of regulatory myosin light chains, which is crucial for muscle and intracellular movement. MLCPs typically contain a catalytic protein phosphatase 1 (PP1c) subunit, a myosin phosphatase targeting (MYPT) subunit, and another smaller subunit. The protein encoded by MYPT3 (protein phosphatase 1 regulatory subunit 16A, PPP1R16A) represents an MYPT subunit, which is responsible for directing PP1c to its intended targets. However, while other MYPTs result in PP1c activation after becoming phosphorylated, the encoded protein is phosphorylated by protein kinase A and then inhibits the catalytic activity of PP1c.

Molecular Weight: 60kD

Gene ID: 84988

UniProt: Q96I34