Src antibody (pTyr529)

Catalog No. ABIN7212926

The Rabbit Polyclonal anti-Src antibody is suitable to detect Src in samples from Human, Mouse, Rat and Monkey. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for Src antibody (pTyr529) (ABIN7212926)

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Src antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Src Antibody

Binding Specificity: pTyr529

Purpose: Rabbit Anti-c-Src (phospho Tyr529) Polyclonal Antibody

Specificity: Phospho-c-Src (Y529) Polyclonal Antibody detects endogenous levels of c-Src protein only when phosphorylated at Y529.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human c-Src Phospho-Tyr529

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Src

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Alternative Name: c-Src

Background: SRC, SRC1, Proto-oncogene tyrosine-protein kinase Src, Proto-oncogene c-Src, pp60c-src, p60-SrcSRC is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. SRC proto-oncogene, non-receptor tyrosine kinase encoded by SRC is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in SRC could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for SRC.

Molecular Weight: 59kD

Gene ID: 6714

UniProt: P12931

Pathways: JAK-STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling