RACGAP1 antibody (pSer387)

Catalog No. ABIN7221368

The Rabbit Polyclonal anti-RACGAP1 antibody is suitable to detect RACGAP1 in samples from Human, Mouse, Rat and Monkey. It has been validated for WB, ELISA, IF and IHC (p).

Quick Overview for RACGAP1 antibody (pSer387) (ABIN7221368)

Target: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RACGAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RACGAP1 Antibody

Binding Specificity: pSer387

Purpose: Rac GAP1 (phospho Ser387) Polyclonal Antibody

Specificity: Phospho-Rac GAP1 (S387) Polyclonal Antibody detects endogenous levels of Rac GAP1 protein only when phosphorylated at S387.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Rac GAP1 Phospho-Ser387

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for RACGAP1

Target: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Alternative Name: Rac GAP1

Background: Rabbit Anti-Rac GAP1 (phospho Ser387) Polyclonal Antibody,RACGAP1, KIAA1478, MGCRACGAP, Rac GTPase-activating protein 1, Male germ cell RacGap, MgcRacGAP, Protein CYK4 homolg, CYK4, HsCYK-4,RACGAP1 encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12.,Rac GTPase-activating protein 1

Molecular Weight: observerd band 72kDa

Gene ID: 29127

UniProt: Q9H0H5

Pathways: Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling of Hepatocyte Growth Factor Receptor