RAD17 antibody

Catalog No. ABIN7216742

The Rabbit Polyclonal anti-RAD17 antibody has been validated for WB, ELISA, IF and IHC (p). It is suitable to detect RAD17 in samples from Human and Mouse.

Quick Overview for RAD17 antibody (ABIN7216742)

Target: RAD17

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD17 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RAD17 Antibody

Purpose: Rad17 Polyclonal Antibody

Specificity: Rad17 Polyclonal Antibody detects endogenous levels of Rad17 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Rad17 around the non-phosphorylation site of S645

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for RAD17

Target: RAD17

Alternative Name: Rad17

Background: Rabbit Anti-Rad17 Polyclonal Antibody,RAD17, R24L, Cell cycle checkpoint protein RAD17, hRad17, RF-C/activator 1 homolog,RAD17 checkpoint clamp loader component encoded by RAD17 is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. RAD17 checkpoint clamp loader component shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. RAD17 checkpoint clamp loader component binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. RAD17 checkpoint clamp loader component recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of RAD17 checkpoint clamp loader component is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of RAD17, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.,Cell cycle checkpoint protein RAD17

Molecular Weight: observerd band 86kDa

Gene ID: 5884

UniProt: O75943