RAD23B antibody (AA 10-90)

Catalog No. ABIN7216746

The Rabbit Polyclonal anti-RAD23B antibody (ABIN7216746) specifically detects RAD23B in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RAD23B antibody (AA 10-90) (ABIN7216746)

Target: RAD23B (RAD23 Homolog B (RAD23B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD23B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-RAD23B Antibody

Binding Specificity: AA 10-90

Purpose: Rabbit Anti-Rad23B Polyclonal Antibody

Specificity: Rad23B Polyclonal Antibody detects endogenous levels of Rad23B protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human Rad23B at AA range: 10-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RAD23B

Target: RAD23B (RAD23 Homolog B (RAD23B))

Alternative Name: Rad23B

Background: RAD23B, UV excision repair protein RAD23 homolog B, HR23B, hHR23B, XP-C repair-complementing complex 58 kDa protein, p58RAD23 homolog B, nucleotide excision repair protein encoded by RAD23B is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). RAD23 homolog B, nucleotide excision repair protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. RAD23 homolog B, nucleotide excision repair protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. RAD23 homolog B, nucleotide excision repair protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus RAD23 homolog B, nucleotide excision repair protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Molecular Weight: 58kD

Gene ID: 5887

UniProt: P54727

Pathways: DNA Damage Repair