RAP1A antibody

Catalog No. ABIN7230934

The Rabbit Polyclonal anti-RAP1A antibody (ABIN7230934) specifically detects RAP1A in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RAP1A antibody (ABIN7230934)

Target: RAP1A (RAP1A, Member of RAS Oncogene Family (RAP1A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAP1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RAP1A Antibody

Purpose: Rabbit Anti-RAP1A Polyclonal Antibody

Specificity: The antibody detects endogenous levels of RAP1A protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human RAP1A protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RAP1A

Target: RAP1A (RAP1A, Member of RAS Oncogene Family (RAP1A))

Alternative Name: RAP1A

Background: C21KG, G-22K, GTP-binding protein smg p21A, Ras-related protein Krev-1RAP1A encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of RAP1A have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants.

Molecular Weight: 20kD

Gene ID: 5906

UniProt: P62834

Pathways: TCR Signaling, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor