RIN1 antibody (AA 630-710)

Catalog No. ABIN7226044

The Rabbit Polyclonal anti-RIN1 antibody (ABIN7226044) specifically detects RIN1 in ELISA, WB and IHC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for RIN1 antibody (AA 630-710) (ABIN7226044)

Target: RIN1 (Ras and Rab Interactor 1 (RIN1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIN1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RIN1 Antibody

Binding Specificity: AA 630-710

Purpose: Rabbit Anti-RIN1 Polyclonal Antibody

Specificity: RIN1 Polyclonal Antibody detects endogenous levels of RIN1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human RIN1 at AA range: 630-710

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300,ELISA 1:2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RIN1

Target: RIN1 (Ras and Rab Interactor 1 (RIN1))

Alternative Name: RIN1

Background: RIN1, Ras and Rab interactor 1, Ras inhibitor JC99, Ras interaction/interference protein 1Ras is a membrane-associated small G protein that is indirectly coupled to receptor and nonreceptor tyrosine kinases. Ras activation is regulated by the levels of bound GTP and GDP. Several effectors of Ras have been identified, including Raf1, PI 3-kinase and RIN1. RIN1 (Ras interaction/interference) was identified as a Ras-interacting protein in yeast, and it has been shown to bind to the human H-Ras. This RIN1-Ras interaction is enhanced when Ras is bound to GTP. Unlike Raf1, RIN1 is localized primarily to the plasma membrane. RIN1 contains an SH2 domain and an amino-terminal region similar to consensus SH3 domains. RIN1 binds c-Abl and, like Raf1, interacts with 14-3-3 proteins.

Molecular Weight: 84kD

Gene ID: 9610

UniProt: Q13671