RCC2 antibody (AA 450-530)

Catalog No. ABIN7223586

The Rabbit Polyclonal anti-RCC2 antibody is suitable to detect RCC2 in samples from Human and Mouse. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for RCC2 antibody (AA 450-530) (ABIN7223586)

Target: RCC2 (Regulator of Chromosome Condensation 2 (RCC2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RCC2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-RCC2 Antibody

Binding Specificity: AA 450-530

Purpose: Rabbit Anti-RCC2 Polyclonal Antibody

Specificity: RCC2 Polyclonal Antibody detects endogenous levels of RCC2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human RCC2 at AA range: 450-530

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RCC2

Target: RCC2 (Regulator of Chromosome Condensation 2 (RCC2))

Alternative Name: RCC2

Background: RCC2, KIAA1470, TD60, Protein RCC2, RCC1-like protein TD-60, Telophase disk protein of 60 kDaRCC2/TD-60 is a member of the RCC1 (regulator of chromosome condensation 1) family of guanine nucleotide exchange factors. RCC2/TD-60 is associated with the chromosome passenger complex (CPC), which also consists of aurora B kinase, borealin, INCENP (inner centromere protein) and survivin. The CPC acts at various stages of mitosis, interacts with microtubules and is required for proper chromosome segregation and cytokinesis. Regulation of aurora B kinase is key in the regulation of the CPC. In late mitosis, RCC2/TD-60 is required for spindle assembly and recruitment of survivin and aurora B. RCC2/TD-60 is also required for aurora B activation in vitro and localization of the CPC to centromeres.

Molecular Weight: 56kD

Gene ID: 55920

UniProt: Q9P258