Ret Proto-Oncogene antibody (pTyr1015)

Catalog No. ABIN7223990

The Rabbit Polyclonal anti-Ret Proto-Oncogene antibody is suitable to detect Ret Proto-Oncogene in samples from Human, Mouse, Rat and Monkey. It has been validated for WB and IHC.

Quick Overview for Ret Proto-Oncogene antibody (pTyr1015) (ABIN7223990)

Target: Ret Proto-Oncogene (RET)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ret Proto-Oncogene antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Ret Proto-Oncogene Antibody

Binding Specificity: pTyr1015

Purpose: Rabbit Anti-Ret (phospho Tyr1015) Polyclonal Antibody

Specificity: Phospho-Ret (Y1015) Polyclonal Antibody detects endogenous levels of Ret protein only when phosphorylated at Y1015.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Ret Phospho-Tyr1015

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Ret Proto-Oncogene

Target: Ret Proto-Oncogene (RET)

Alternative Name: Ret

Background: RET, CDHF12, CDHR16, PTC, RET51, Proto-oncogene tyrosine-protein kinase receptor Ret, Cadherin family member 12, Proto-oncogene c-RetRET (ret proto-oncogene), a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. RET plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in RET are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for RET. Additional transcript variants have been described but their biological validity has not been confirmed.

Molecular Weight: 124kD

Gene ID: 5979

UniProt: P07949

Pathways: RTK Signaling, Dopaminergic Neurogenesis, Regulation of Cell Size, Tube Formation