ARHGAP4 antibody (AA 890-970)

Catalog No. ABIN7216342

The Rabbit Polyclonal anti-ARHGAP4 antibody is suitable to detect ARHGAP4 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for ARHGAP4 antibody (AA 890-970) (ABIN7216342)

Target: ARHGAP4 (rho GTPase Activating Protein 4 (ARHGAP4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGAP4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ARHGAP4 Antibody

Binding Specificity: AA 890-970

Purpose: Rabbit Anti-p115 Polyclonal Antibody

Specificity: P115 Polyclonal Antibody detects endogenous levels of p115 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human p115 at AA range: 890-970

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ARHGAP4

Target: ARHGAP4 (rho GTPase Activating Protein 4 (ARHGAP4))

Alternative Name: p115

Background: USO1, VDP, General vesicular transport factor p115, Protein USO1 homolog, Transcytosis-associated protein, TAP, Vesicle-docking proteinUSO1 vesicle transport factor encoded by USO1 is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits USO1 vesicle transport factor to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants.

Molecular Weight: 108kD

Gene ID: 8615

UniProt: O60763

Pathways: Neurotrophin Signaling Pathway, Regulation of Cell Size