RPL14 antibody (AA 40-120)

Catalog No. ABIN7225778

The Rabbit Polyclonal anti-RPL14 antibody is suitable to detect RPL14 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IHC.

Quick Overview for RPL14 antibody (AA 40-120) (ABIN7225778)

Target: RPL14 (Ribosomal Protein L14 (RPL14))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPL14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-RPL14 Antibody

Binding Specificity: AA 40-120

Purpose: Rabbit Anti-Ribosomal Protein L14 Polyclonal Antibody

Specificity: Ribosomal Protein L14 Polyclonal Antibody detects endogenous levels of Ribosomal Protein L14 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Ribosomal Protein L14 at AA range: 40-120

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300,ELISA 1:2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RPL14

Target: RPL14 (Ribosomal Protein L14 (RPL14))

Alternative Name: Ribosomal Protein L14

Background: RPL14, 60S ribosomal protein L14, CAG-ISL 7Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL14 (ribosomal protein L14) encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. RPL14 contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic, these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5'-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPL14 dispersed through the genome.

Molecular Weight: 26kD

Gene ID: 9045

UniProt: P50914