SKP1 antibody (AA 10-90)

Catalog No. ABIN7217022

The Rabbit Polyclonal anti-SKP1 antibody (ABIN7217022) specifically detects SKP1 in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for SKP1 antibody (AA 10-90) (ABIN7217022)

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SKP1 Antibody

Binding Specificity: AA 10-90

Purpose: Rabbit Anti-Skp1 p19 Polyclonal Antibody

Specificity: Skp1 p19 Polyclonal Antibody detects endogenous levels of Skp1 p19 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Skp1 p19 at AA range: 10-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SKP1

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Alternative Name: Skp1 p19

Background: SKP1, EMC19, OCP2, SKP1A, TCEB1L, S-phase kinase-associated protein 1, Cyclin-A/CDK2-associated protein p19, Organ of Corti protein 2, OCP-2, Organ of Corti protein II, OCP-II, RNA polymerase II elongation factor-like protein, SIII, TranscrSKP1 encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. S-phase kinase-associated protein 1 binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.

Molecular Weight: 19kD

Gene ID: 6500

UniProt: P63208

Pathways: Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases