Osteopontin antibody (AA 230-310)

Catalog No. ABIN7216326

The Rabbit Polyclonal anti-Osteopontin antibody (ABIN7216326) specifically detects Osteopontin in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Osteopontin antibody (AA 230-310) (ABIN7216326)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Osteopontin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Osteopontin Antibody

Binding Specificity: AA 230-310

Purpose: Rabbit Anti-OPN Polyclonal Antibody

Specificity: OPN Polyclonal Antibody detects endogenous levels of OPN protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human OPN at AA range: 230-310

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:100-500,IF/ICC1:100-500,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Osteopontin

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Alternative Name: OPN

Background: SPP1, BNSP, OPN, PSEC0156, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, UropontinSecreted phosphoprotein 1 encoded by SPP1 is involved in the attachment of osteoclasts to the mineralized bone matrix. Secreted phosphoprotein 1 is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to secreted phosphoprotein 1. Secreted phosphoprotein 1 is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for SPP1.

Molecular Weight: 60kD

Gene ID: 6696

UniProt: P10451

Pathways: Regulation of Cell Size