SPTLC1 antibody (C-Term)

Catalog No. ABIN7218000

This Rabbit Polyclonal antibody specifically detects SPTLC1 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SPTLC1 antibody (C-Term) (ABIN7218000)

Target: SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPTLC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-SPTLC1 Antibody

Binding Specificity: C-Term

Purpose: Rabbit Anti-SPTLC1 Polyclonal Antibody

Specificity: SPTLC1 Polyclonal Antibody detects endogenous levels of SPTLC1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human SPTLC1

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC: 1:100-300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SPTLC1

Target: SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))

Alternative Name: SPTLC1

Background: SPTLC1, LCB1, Serine palmitoyltransferase 1, Long chain base biosynthesis protein 1, LCB 1, Serine-palmitoyl-CoA transferase 1, SPT 1, SPT1SPTLC1 encodes a member of the class-II pyridoxal-phosphate-dependent aminotransferase family. The serine palmitoyltransferase long chain base subunit 1 is the long chain base subunit 1 of serine palmitoyltransferase. Serine palmitoyltransferase converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate and is the key enzyme in sphingolipid biosynthesis. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified. Pseudogenes of this gene have been defined on chromosomes 1, 6, 10, and 13.

Molecular Weight: 52kD

Gene ID: 10558

UniProt: O15269