SGK1 antibody (pSer422)

Catalog No. ABIN7224278

This Rabbit Polyclonal antibody specifically detects SGK1 in WB, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SGK1 antibody (pSer422) (ABIN7224278)

Target: SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SGK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SGK1 Antibody

Binding Specificity: pSer422

Purpose: SGK1 (phospho Ser422) Polyclonal Antibody

Specificity: Phospho-SGK1 (S422) Polyclonal Antibody detects endogenous levels of SGK1 protein only when phosphorylated at S422.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human SGK1 Phospho-Ser422

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for SGK1

Target: SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))

Alternative Name: SGK1

Background: Rabbit Anti-SGK1 (phospho Ser422) Polyclonal Antibody,SGK1, SGK, Serine/threonine-protein kinase Sgk1, Serum/glucocorticoid-regulated kinase 1,SGK1 (serum/glucocorticoid regulated kinase 1) encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of SGK1 may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for SGK1.,serine threonine-protein kinase Sgk1

Molecular Weight: observerd band 57kDa

Gene ID: 6446

UniProt: O00141

Pathways: MAPK Signaling, Notch Signaling, Steroid Hormone Mediated Signaling Pathway