SHC1 antibody

Catalog No. ABIN7216998

This anti-SHC1 antibody is a Rabbit Polyclonal antibody detecting SHC1 in WB, ELISA and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for SHC1 antibody (ABIN7216998)

Target: SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SHC1 Antibody

Purpose: Shc Polyclonal Antibody

Specificity: Shc Polyclonal Antibody detects endogenous levels of Shc protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Shc around the non-phosphorylation site of Y427

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for SHC1

Target: SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))

Alternative Name: Shc

Background: Rabbit Anti-Shc Polyclonal Antibody,SHC1, SHC, SHCA, SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1, SH2 domain protein C1,SHC1 encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.,SHC-transforming protein 1

Molecular Weight: observerd band 66(p66 isoform), 52(p52 isoform), 46(p46 isoform)kDa

Gene ID: 6464

UniProt: P29353

Pathways: RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, ER-Nucleus Signaling, Signaling Events mediated by VEGFR1 and VEGFR2