Shugoshin antibody (AA 240-320)

Catalog No. ABIN7216994

The Rabbit Polyclonal anti-Shugoshin antibody (ABIN7216994) specifically detects Shugoshin in ELISA, WB, IHC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for Shugoshin antibody (AA 240-320) (ABIN7216994)

Target: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Shugoshin antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Shugoshin Antibody

Binding Specificity: AA 240-320

Purpose: Rabbit Anti-Sgo1 Polyclonal Antibody

Specificity: Sgo1 Polyclonal Antibody detects endogenous levels of Sgo1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Sgo1 at AA range: 240-320

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Shugoshin

Target: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Alternative Name: Sgo1

Background: SGOL1, SGO1, Shugoshin-like 1, hSgo1, Serologically defined breast cancer antigen NY-BR-85Shugoshin-like 1 encoded by SGOL1 is a member of the shugoshin family of proteins. This protein is thought to protect centromeric cohesin from cleavage during mitotic prophase by preventing phosphorylation of a cohesin subunit. Reduced expression of this gene leads to the premature loss of centromeric cohesion, mis-segregation of sister chromatids, and mitotic arrest. Evidence suggests that this protein also protects a small subset of cohesin found along the length of the chromosome arms during mitotic prophase. An isoform lacking exon 6 has been shown to play a role in the cohesion of centrioles (PMID: 16582621 and PMID: 18331714). Mutations in this gene have been associated with Chronic Atrial and Intestinal Dysrhythmia (CAID) syndrome, characterized by the co-occurrence of Sick Sinus Syndrome (SSS) and Chronic Intestinal Pseudo-obstruction (CIPO) within the first four decades of life (PMID: 25282101). Fibroblast cells from CAID patients exhibited both increased cell proliferation and higher rates of senescence. Pseudogenes of this gene have been found on chromosomes 1 and 7. Alternative splicing results in multiple transcript variants.

Molecular Weight: 64kD

Gene ID: 151648

UniProt: Q5FBB7

Pathways: Maintenance of Protein Location