SGOL2 antibody (AA 650-730)

Catalog No. ABIN7219846

The Rabbit Polyclonal anti-SGOL2 antibody is suitable to detect SGOL2 in samples from Human and Mouse. It has been validated for ELISA, IHC and IF.

Quick Overview for SGOL2 antibody (AA 650-730) (ABIN7219846)

Target: SGOL2 (Shugoshin-Like 2 (SGOL2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SGOL2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SGOL2 Antibody

Binding Specificity: AA 650-730

Purpose: Rabbit Anti-Sgo2 Polyclonal Antibody

Specificity: Sgo2 Polyclonal Antibody detects endogenous levels of Sgo2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Sgo2 at AA range: 650-730

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SGOL2

Target: SGOL2 (Shugoshin-Like 2 (SGOL2))

Alternative Name: Sgo2

Background: SGOL2, Shugoshin-like 2, Shugoshin-2, Sgo2, TripinCooperates with PPP2CA to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Has a crucial role in protecting REC8 at centromeres from cleavage by separase. During meiosis, protects centromeric cohesion complexes until metaphase II/anaphase II transition, preventing premature release of meiosis-specific REC8 cohesin complexes from anaphase I centromeres. Is thus essential for an accurate gametogenesis. May act by targeting PPP2CA to centromeres, thus leading to cohesin dephosphorylation (By similarity). Essential for recruiting KIF2C to the inner centromere and for correcting defective kinetochore attachments.

Gene ID: 151246

UniProt: Q562F6