SIRT3 antibody (AA 320-400)

Catalog No. ABIN7220710

The Rabbit Polyclonal anti-SIRT3 antibody (ABIN7220710) specifically detects SIRT3 in WB and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for SIRT3 antibody (AA 320-400) (ABIN7220710)

Target: SIRT3 (Sirtuin 3 (SIRT3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SIRT3 Antibody

Binding Specificity: AA 320-400

Purpose: Rabbit Anti-SIRT3 Polyclonal Antibody

Specificity: SIRT3 Polyclonal Antibody detects endogenous levels of SIRT3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human SIRT3 at AA range: 320-400

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SIRT3

Target: SIRT3 (Sirtuin 3 (SIRT3))

Alternative Name: SIRT3

Background: SIRT3, SIR2L3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, hSIRT3, Regulatory protein SIR2 homolog 3, SIR2-like protein 3SIRT3 encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The sirtuin 3 encoded by SIRT3 is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.

Molecular Weight: 45kD

Gene ID: 23410

UniProt: Q9NTG7