SMAD7 antibody

Catalog No. ABIN7231402

The Rabbit Polyclonal anti-SMAD7 antibody (ABIN7231402) specifically detects SMAD7 in WB and ELISA. The antibody is reactive with Human, Mouse, Rat, Goat and Sheep samples.

Quick Overview for SMAD7 antibody (ABIN7231402)

Target: SMAD7 (SMAD, Mothers Against DPP Homolog 7 (SMAD7))

Reactivity: Human, Mouse, Rat, Goat, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SMAD7 Antibody

Purpose: Rabbit Anti-SMAD7 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of SMAD7 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human SMAD7 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SMAD7

Target: SMAD7 (SMAD, Mothers Against DPP Homolog 7 (SMAD7))

Alternative Name: SMAD7

Background: Mothers against decapentaplegic homolog 7, MAD homolog 7, Mothers against DPP homolog 7, Mothers against decapentaplegic homolog 8, MAD homolog 8, Mothers against DPP homolog 8, SMAD family member 7, SMAD 7, Smad7, hSMAD7The protein encoded by SMAD7 is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of SMAD7 is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for SMAD7.

Molecular Weight: 46kD

Gene ID: 4092

UniProt: O15105

Pathways: Interferon-gamma Pathway, Cell-Cell Junction Organization