SP100 antibody (AA 250-330)

Catalog No. ABIN7224440

The Rabbit Polyclonal anti-SP100 antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect SP100 in samples from Human.

Quick Overview for SP100 antibody (AA 250-330) (ABIN7224440)

Target: SP100 (SP100 Nuclear Antigen (SP100))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SP100 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SP100 Antibody

Binding Specificity: AA 250-330

Purpose: SP-100 Polyclonal Antibody

Specificity: SP-100 Polyclonal Antibody detects endogenous levels of SP-100 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human SP-100 at AA range: 250-330

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for SP100

Target: SP100 (SP100 Nuclear Antigen (SP100))

Alternative Name: SP-100

Background: Rabbit Anti-SP-100 Polyclonal Antibody,SP100, Nuclear autoantigen Sp-100, Lysp100b, Nuclear dot-associated Sp100 protein, Speckled 100 kDa,SP100 (SP100 nuclear antigen) encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for SP100, one of which encodes a high-mobility group protein.,Nuclear autoantigen Sp-100

Molecular Weight: observerd band 100kDa

Gene ID: 6672

UniProt: P23497

Pathways: Retinoic Acid Receptor Signaling Pathway