SPTAN1 antibody (Cleaved-Asp1185)

Catalog No. ABIN7212504

The Rabbit Polyclonal anti-SPTAN1 antibody is suitable to detect SPTAN1 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for SPTAN1 antibody (Cleaved-Asp1185) (ABIN7212504)

Target: SPTAN1 (Spectrin alpha Chain, Brain (SPTAN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPTAN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SPTAN1 Antibody

Binding Specificity: AA 1110-1190, Cleaved-Asp1185

Purpose: Rabbit Anti-Cleaved-Spectrin α II (D1185) Polyclonal Antibody

Specificity: Cleaved-Spectrin α II (D1185) Polyclonal Antibody detects endogenous levels of fragment of activated Spectrin α II protein resulting from cleavage adjacent to D1185.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Spectrin alpha II at AA range: 1110-1190

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SPTAN1

Target: SPTAN1 (Spectrin alpha Chain, Brain (SPTAN1))

Alternative Name: Spectrin alpha II

Background: SPTAN1, NEAS, SPTA2, Spectrin alpha chain, non-erythrocytic 1, Alpha-II spectrin, Fodrin alpha chain, Spectrin, non-erythroid alpha subunitSpectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. SPTAN1 encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. Spectrin alpha chain non-erythrocytic 1 has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in SPTAN1 are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.

Molecular Weight: 137kD

Gene ID: 6709

UniProt: Q13813

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization