SF3B1 antibody (N-Term)

Catalog No. ABIN7217628

The Rabbit Polyclonal anti-SF3B1 antibody is suitable to detect SF3B1 in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for SF3B1 antibody (N-Term) (ABIN7217628)

Target: SF3B1 (Splicing Factor 3b, Subunit 1, 155kDa (SF3B1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SF3B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SF3B1 Antibody

Binding Specificity: N-Term

Purpose: SAP 155 Polyclonal Antibody

Specificity: SAP 155 Polyclonal Antibody detects endogenous levels of SAP 155 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the N-terminal region of human SAP 155

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for SF3B1

Target: SF3B1 (Splicing Factor 3b, Subunit 1, 155kDa (SF3B1))

Alternative Name: SAP 155

Background: Rabbit Anti-SAP 155 Polyclonal Antibody,SF3B1, SAP155, Splicing factor 3B subunit 1, Pre-mRNA-splicing factor SF3b 155 kDa subunit, SF3b155, Spliceosome-associated protein 155, SAP 155,SF3B1 encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.,Splicing factor 3B subunit 1

Molecular Weight: observerd band 145kDa

Gene ID: 23451

UniProt: O75533

Pathways: Chromatin Binding