SREBF1 antibody (pSer439)

Catalog No. ABIN7224464

The Rabbit Polyclonal anti-SREBF1 antibody (ABIN7224464) specifically detects SREBF1 in WB, ELISA, IHC and IF. The antibody is reactive with Human and Rat samples.

Quick Overview for SREBF1 antibody (pSer439) (ABIN7224464)

Target: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SREBF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SREBF1 Antibody

Binding Specificity: pSer439

Purpose: Rabbit Anti-SREBP-1 (phospho Ser439) Polyclonal Antibody

Specificity: Phospho-SREBP-1 (S439) Polyclonal Antibody detects endogenous levels of SREBP-1 protein only when phosphorylated at S439.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human SREBP-1 Phospho-Ser439

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SREBF1

Target: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Alternative Name: SREBP-1

Background: SREBF1, BHLHD1, SREBP1, Sterol regulatory element-binding protein 1, SREBP-1, Class D basic helix-loop-helix protein 1, bHLHd1, Sterol regulatory element-binding transcription factor 1SREBF1 (sterol regulatory element binding transcription factor 1) encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. SREBF1 is located within the Smith-Magenis syndrome region on chromosome 17.

Molecular Weight: 122kD

Gene ID: 6720

UniProt: P36956

Pathways: AMPK Signaling, Caspase Cascade in Apoptosis, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha