14-3-3 sigma/SFN antibody (AA 10-90)

Catalog No. ABIN7221170

The Rabbit Polyclonal anti-14-3-3 sigma/SFN antibody (ABIN7221170) specifically detects 14-3-3 sigma/SFN in WB, IHC, ELISA and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for 14-3-3 sigma/SFN antibody (AA 10-90) (ABIN7221170)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 sigma/SFN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-14-3-3 sigma/SFN Antibody

Binding Specificity: AA 10-90

Purpose: Rabbit Anti-14-3-3 σ Polyclonal Antibody

Specificity: 14-3-3 σ Polyclonal Antibody detects endogenous levels of 14-3-3 σ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human 14-3-3 sigma at AA range: 10-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC: 100-300,WB 1:500-1:2000,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name: 14-3-3 sigma

Background: SFN, HME1, 14-3-3 protein sigma, Epithelial cell marker protein 1, StratifinSFN (Stratifin) is a Protein Coding gene. Diseases associated with SFN include pancreatic ductal adenocarcinoma and limbal stem cell deficiency. Among its related pathways are Gene Expression and Signaling by GPCR. GO annotations related to this gene include protein kinase binding and phosphoprotein binding. An important paralog of this gene is YWHAZ. dapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53. 53-regulated inhibitor of G2/M progression.

Molecular Weight: 30kD

Gene ID: 2810

UniProt: P31947

Pathways: p53 Signaling, Myometrial Relaxation and Contraction