TERT antibody (pSer227)

Catalog No. ABIN7224664

The Rabbit Polyclonal anti-TERT antibody (ABIN7224664) specifically detects TERT in ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for TERT antibody (pSer227) (ABIN7224664)

Target: TERT (Telomerase Reverse Transcriptase (TERT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TERT antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Product Details anti-TERT Antibody

Binding Specificity: pSer227

Purpose: Rabbit Anti-TERT (phospho Ser227) Polyclonal Antibody

Specificity: Phospho-TERT (S227) Polyclonal Antibody detects endogenous levels of TERT protein only when phosphorylated at S227.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human TERT Phospho-Ser227

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications,

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TERT

Target: TERT (Telomerase Reverse Transcriptase (TERT))

Alternative Name: TERT

Background: TERT, EST2, TCS1, TRT, Telomerase reverse transcriptase, HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2, TP2Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified, the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity.

Gene ID: 7015

UniProt: O14746

Pathways: Telomere Maintenance