THRB antibody (pSer142)

Catalog No. ABIN7224696

This Rabbit Polyclonal antibody specifically detects THRB in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for THRB antibody (pSer142) (ABIN7224696)

Target: THRB (Thyroid Hormone Receptor, beta (THRB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This THRB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-THRB Antibody

Binding Specificity: pSer142

Purpose: Rabbit Anti-TRβ1 (phospho Ser142) Polyclonal Antibody

Specificity: Phospho-TRβ1 (S142) Polyclonal Antibody detects endogenous levels of TRβ1 protein only when phosphorylated at S142.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human TRbeta1 Phospho-Ser142

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for THRB

Target: THRB (Thyroid Hormone Receptor, beta (THRB))

Alternative Name: TRbeta1

Background: THRB, ERBA2, NR1A2, THR1, Thyroid hormone receptor beta, Nuclear receptor subfamily 1 group A member 2, c-erbA-2, c-erbA-betaThe protein encoded by THRB (thyroid hormone receptor beta) is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Mutations in THRB are known to be a cause of generalized thyroid hormone resistance (GTHR), a syndrome characterized by goiter and high levels of circulating thyroid hormone (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH). Several alternatively spliced transcript variants encoding the same protein have been observed for THRB.

Molecular Weight: 45kD

Gene ID: 7068

UniProt: P10828

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Sensory Perception of Sound