TOP3A antibody (AA 350-430)

Catalog No. ABIN7226600

The Rabbit Polyclonal anti-TOP3A antibody has been validated for WB and ELISA. It is suitable to detect TOP3A in samples from Human and Mouse.

Quick Overview for TOP3A antibody (AA 350-430) (ABIN7226600)

Target: TOP3A (Topoisomerase (DNA) III alpha (TOP3A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOP3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TOP3A Antibody

Binding Specificity: AA 350-430

Purpose: Topo IIIα Polyclonal Antibody

Specificity: Topo IIIα Polyclonal Antibody detects endogenous levels of Topo IIIα protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Topo IIIalpha at AA range: 350-430

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for TOP3A

Target: TOP3A (Topoisomerase (DNA) III alpha (TOP3A))

Alternative Name: Topo IIIalpha

Background: Rabbit Anti-Topo IIIα Polyclonal Antibody,TOP3A, TOP3, DNA topoisomerase 3-alpha, DNA topoisomerase III alpha,TOP3A(topoisomerase(DNA) III alpha) encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. Alternative splicing results in multiple transcript variants encoding different isoforms.,DNA topoisomerase 3-alpha

Molecular Weight: observerd band 115kDa

Gene ID: 7156

UniProt: Q13472

Pathways: DNA Damage Repair