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TOP3A antibody

TOP3A Reactivity: Human, Mouse, Rat WB, ELISA, IP Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7120017
  • Target See all TOP3A Antibodies
    TOP3A (Topoisomerase (DNA) III alpha (TOP3A))
    Reactivity
    • 6
    • 3
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 6
    Rabbit
    Clonality
    • 6
    Polyclonal
    Conjugate
    • 6
    This TOP3A antibody is un-conjugated
    Application
    • 6
    • 4
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Purification
    Immunogen affinity purified
    Purity
    ≥95 % as determined by SDS-PAGE
    Immunogen
    topoisomerase(DNA) III alpha
    Isotype
    IgG
    Top Product
    Discover our top product TOP3A Primary Antibody
  • Application Notes
    WB: 1:500-1:2000, IP: 1:200-1:2000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
    Expiry Date
    12 months
  • Target
    TOP3A (Topoisomerase (DNA) III alpha (TOP3A))
    Alternative Name
    TOP3A (TOP3A Products)
    Synonyms
    TOP3 antibody, DNA topoisomerase III alpha antibody, topoisomerase (DNA) III alpha antibody, Top3a antibody, TOP3A antibody
    Background
    Synonyms:TOP3 Background:Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity.
    Molecular Weight
    100-110 kDa
    Gene ID
    7156
    UniProt
    Q13472
    Pathways
    DNA Damage Repair
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