TFAM antibody (AA 100-180)

Catalog No. ABIN7224670

The Rabbit Polyclonal anti-TFAM antibody is suitable to detect TFAM in samples from Human, Mouse, Rat and Cow. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for TFAM antibody (AA 100-180) (ABIN7224670)

Target: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TFAM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-TFAM Antibody

Binding Specificity: AA 100-180

Purpose: Rabbit Anti-mtTFA Polyclonal Antibody

Specificity: MtTFA Polyclonal Antibody detects endogenous levels of mtTFA protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human mtTFA at AA range: 100-180

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TFAM

Target: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Alternative Name: mtTFA

Background: TFAM, TCF6, TCF6L2, Transcription factor A, mitochondrial, mtTFA, Mitochondrial transcription factor 1, MtTF1, Transcription factor 6, TCF-6, Transcription factor 6-like 2TFAM (transcription factor A, mitochondrial) encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in TFAM are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for TFAM on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants.

Molecular Weight: 22kD

Gene ID: 7019

UniProt: Q00059

Pathways: Chromatin Binding