Tryptophan Hydroxylase 1 antibody (pSer58)

Catalog No. ABIN7224798

The Rabbit Polyclonal anti-Tryptophan Hydroxylase 1 antibody is suitable to detect Tryptophan Hydroxylase 1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for Tryptophan Hydroxylase 1 antibody (pSer58) (ABIN7224798)

Target: Tryptophan Hydroxylase 1 (TPH1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tryptophan Hydroxylase 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Tryptophan Hydroxylase 1 Antibody

Binding Specificity: pSer58

Purpose: Rabbit Anti-TPH1 (phospho Ser58) Polyclonal Antibody

Specificity: Phospho-TPH1 (S58) Polyclonal Antibody detects endogenous levels of TPH1 protein only when phosphorylated at S58.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human TPH1 Phospho-Ser58

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Tryptophan Hydroxylase 1

Target: Tryptophan Hydroxylase 1 (TPH1)

Alternative Name: TPH1

Background: TPH1, TPH, TPRH, TRPH, Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase 1TPH1 (tryptophan hydroxylase 1) encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in TPH1 have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.

Molecular Weight: 51kD

Gene ID: 7166

UniProt: P17752

Pathways: Feeding Behaviour