Tuberin antibody (pThr1462)

Catalog No. ABIN7224812

The Rabbit Polyclonal anti-Tuberin antibody has been validated for ELISA, WB, IF and IHC (p). It is suitable to detect Tuberin in samples from Human, Mouse and Rat.

Quick Overview for Tuberin antibody (pThr1462) (ABIN7224812)

Target: Tuberin (TSC2) (Tuberous Sclerosis 2 (TSC2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tuberin antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Tuberin Antibody

Binding Specificity: pThr1462

Purpose: Tuberin (phospho Thr1462) Polyclonal Antibody

Specificity: Phospho-Tuberin (T1462) Polyclonal Antibody detects endogenous levels of Tuberin protein only when phosphorylated at T1462.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Tuberin Phospho-Thr1462

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Tuberin

Target: Tuberin (TSC2) (Tuberous Sclerosis 2 (TSC2))

Alternative Name: Tuberin

Background: Rabbit Anti-Tuberin (phospho Thr1462) Polyclonal Antibody,TSC2, TSC4, Tuberin, Tuberous sclerosis 2 protein,Mutations in TSC2 (tuberous sclerosis 2) lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms.,Tuberin

Molecular Weight: observerd band 200kDa

Gene ID: 7249

UniProt: P49815

Pathways: RTK Signaling, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus