VRK1 antibody

Catalog No. ABIN7232118

The Rabbit Polyclonal anti-VRK1 antibody (ABIN7232118) specifically detects VRK1 in WB and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for VRK1 antibody (ABIN7232118)

Target: VRK1 (Vaccinia Related Kinase 1 (VRK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VRK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-VRK1 Antibody

Purpose: Rabbit Anti-VRK1 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of VRK1 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human VRK1 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for VRK1

Target: VRK1 (Vaccinia Related Kinase 1 (VRK1))

Alternative Name: VRK1

Background: Serine/threonine-protein kinase VRK1, Vaccinia-related kinase 1VRK1 encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. VRK1 is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 Molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. VRK1, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.

Molecular Weight: 43kD

Gene ID: 7443

UniProt: Q99986